| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
05/01/2001 |
|
Data da última atualização: |
05/01/2001 |
|
Autoria: |
SMIT, M. P.; SCHOLTENS, W.; KRUGER, W. J. |
|
Afiliação: |
Drs. M. P. Smit, W. Scholtens en W. J. Kruger; Vleeskeuringsdienst "Kring Groningen", Zaagmuldersweg 1-2, Groningen. |
|
Título: |
Sterilisatie van plastic-cultuurdoosjes door middel van Formaldehydedamp. |
|
Ano de publicação: |
1977 |
|
Fonte/Imprenta: |
Tyjdschr. Diergeneesk., v.102, n.19, p.1129-1137, 1977. |
|
Idioma: |
Outros |
|
Conteúdo: |
Various experiments showed that plastic culture boxes may be sterilized by formaldehyde vapour in an exsiccator. Detectable quanties of formaldehyde were no longer found to be present when the dishes had been stored at room temperature for 24 hours after sterilisation. A striking fature in these experiments consisted in the fact that colonies failed to grow on those agar plates, the surfaces of which had been in contact with formaldehyde vapour. The dishes used should be meticulously cleaned as studies showed that complete sterilization was not achieved when bacteria were present in or on agar. When Petri dishes cleanced in alhocol and then sterilised were used in assessing aerobic bacterial counts in minced meat by the mixed plate technique, significantly higher average numbers of colonies were recorded than was the case when commercially available sterilized Petri dishes were used. When the streak plate method was adopted, no significant difference were observed.When all the required manoeuvres are performed with meticulous care, the method is a practicable one ensuring that plastic culture boxes may be used again several times. |
|
Palavras-Chave: |
Bacterias; Biology; Laboratory practice; Pratica de laboratorio. |
|
Thesagro: |
Biologia. |
|
Thesaurus Nal: |
bacterial spores. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01742naa a2200217 a 4500
001 1649819
005 2001-01-05
008 1977 bl --- 0-- u #d
100 1 $aSMIT, M. P.
245 $aSterilisatie van plastic-cultuurdoosjes door middel van Formaldehydedamp.
260 $c1977
520 $aVarious experiments showed that plastic culture boxes may be sterilized by formaldehyde vapour in an exsiccator. Detectable quanties of formaldehyde were no longer found to be present when the dishes had been stored at room temperature for 24 hours after sterilisation. A striking fature in these experiments consisted in the fact that colonies failed to grow on those agar plates, the surfaces of which had been in contact with formaldehyde vapour. The dishes used should be meticulously cleaned as studies showed that complete sterilization was not achieved when bacteria were present in or on agar. When Petri dishes cleanced in alhocol and then sterilised were used in assessing aerobic bacterial counts in minced meat by the mixed plate technique, significantly higher average numbers of colonies were recorded than was the case when commercially available sterilized Petri dishes were used. When the streak plate method was adopted, no significant difference were observed.When all the required manoeuvres are performed with meticulous care, the method is a practicable one ensuring that plastic culture boxes may be used again several times.
650 $abacterial spores
650 $aBiologia
653 $aBacterias
653 $aBiology
653 $aLaboratory practice
653 $aPratica de laboratorio
700 1 $aSCHOLTENS, W.
700 1 $aKRUGER, W. J.
773 $tTyjdschr. Diergeneesk.$gv.102, n.19, p.1129-1137, 1977.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 1 | |
| Registros recuperados : 1 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
